mouse igg2b  (Developmental Studies Hybridoma Bank)

Journal: bioRxiv

Article Title: Genetic Network Shaping Kenyon Cell Identity and Function in Drosophila Mushroom Bodies

doi: 10.1101/2025.07.07.663451

Figure Lengend Snippet: E93 is sufficient to shift the KC identity towards α / β neural-like fate (A-B) Overexpression of E93 driven by GAL4-OK107 caused precocious expression of α/β-specific Ca-α1T-GFSTF in early-born KCs at the WL stage. (C-D) In addition, overexpression of E93 driven by a γ-neural driver, GAL4-201Y (magenta), ectopically turned on the expression of a α/β-specific 70F05-LexA driver in a portion of γ neurons (visualized by myr-GFP in green; arrow). (E-J) On the other hand, overexpression of E93 abolished γ-specific markers, including Ab-GFP (E-F), Mamo H/I (G-H), Mamo D∼G (weak green signal; I-J) and EcR-B1 (E-J), and α’/β’-specific Mamo D∼G (strong green signal within yellow dashed-line; I-J) in the early-born KCs at the white pupal (WP) stage. (K-L) E93 overexpression also compromised the Lac-FSVS expression in α’/β’ neurons and the morphology of MB lobes revealed by cell adhesion molecule Fasciclin II (Fas2, strong magenta for labeling α and β lobes) at 24 h after puparium formation (APF). An enhance-promoter (EP) line inserted at the proximal region of the E93-A 5’UTR was used to overexpress E93 in the gain-of-function experiments. The potency of the E93(EP) line was similar to two other in-house transgenic lines expressing E93-A and E93-B isoforms (see S9 Fig). Scale bar: 10 µm.

Article Snippet: Primary antibodies used in this study included guinea pig antibody against Chinmo (1:1000, Sokol laboratory [ ]), rat monoclonal antibody against mCD8 (1:100, Thermo Fisher Scientific), rabbit antibody against GFP (1:750, Thermo Fisher Scientific), and mouse monoclonal antibodies against EcR-B1 (1:50, DSHB), Fas2 (1:100, DSHB) and Trio (1:50, DSHB).

Techniques: Over Expression, Expressing, Labeling, Transgenic Assay

Journal: eLife

Article Title: The function of juvenile–adult transition axis in female sexual receptivity of Drosophila melanogaster

doi: 10.7554/eLife.92545

Figure Lengend Snippet:

Article Snippet: Antibody , Anti- Drosophila ecdysone receptor (EcR-B1), mouse monoclonal , Developmental Studies Hybridoma Bank , Cat# AD4.4(EcR-B1), RRID: AB_2154902 , IHC (1:10).

Techniques: Electron Microscopy, Recombinant, Software